Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: Effects of HGF regulated by autophagy on antifibrotic function of fPMSCs. The conditioned medium from sh-NC and sh-ATG5 infection of fPMSCs were infected with sh-NC and sh-ATG5 lentivirus and pretreated with 20 ng/mL TGF-β1 in order to prepare conditioned medium, then which was conducted to culture MRC-5 cells stimulated with 2.5 ng/mL TGF-β1 for 24 h. 500 ng/mL recombinant human HGF (rhHGF) and 1 μg/mL HGF neutralization antibody (naHGF) was added into conditioned medium respectively to interrupt the function of HGF. Protein levels of Collagen I, α-SMA and CTGF were detected by western blotting. Densitometric analyses of Collagen I, α-SMA and CTGF. Representative data from three independent experiments are shown. * P < 0.05.

Article Snippet: A coculture system was treated with 500 ng/mL recombinant human HGF (Proteintech, China) and 1 μg/mL HGF neutralization antibody (R&D Systems, USA) to verify the antifibrotic function of HGF derived from fPMSCs on MRC-5 cells.

Techniques: Infection, Recombinant, Neutralization, Western Blot